human oc cell lines  (ATCC)

Journal: Experimental and Therapeutic Medicine

Article Title: Long non-coding RNA KCNQ1OT1 promotes ovarian cancer cell malignant characteristics by targeting the miR-140-5p/KLK10 axis

doi: 10.3892/etm.2026.13129

Figure Lengend Snippet: Expression of target genes in OC datasets and cell lines. Principal component analysis plots showing expression patterns in the (A) GSE66957 and (C) GSE47841 datasets. Volcano plots illustrating differentially expressed genes in the (B) GSE66957 and (D) GSE47841 datasets. Differential expression of (E) KCNQ1OT1 ( GSE66957 ), (F) KLK10 ( GSE66957 ) and (G) miR-140-5p ( GSE47841 ). (H) Relative expression levels of KCNQ1OT1, KLK10 and miR-140-5p in SKOV3 OC cells and IOSE80 normal ovarian epithelial cells. * P<0.05 and ** P<0.01. OC, ovarian cancer; KCNQ1OT1, potassium voltage-gated channel subfamily Q member 1 opposite strand/antisense transcript 1; KLK10, kallikrein-related peptidase 10; miR, microRNA; Dim, dimension; FC, fold change.

Article Snippet: IOSE80 (normal ovarian epithelial) and SKOV3 (OC) cell lines were sourced from Procell Life Science & Technology Co., Ltd. IOSE80 cells were maintained in DMEM (Gibco; Thermo Fisher Scientific, Inc.) and SKOV3 cells in McCoy's 5A medium (Gibco; Thermo Fisher Scientific, Inc.), both supplemented with 10% FBS (HyClone; Cytiva) and 1% penicillin-streptomycin, at 37 ̊C in a 5% CO 2 humidified incubator.

Techniques: Expressing, Quantitative Proteomics

Journal: PLOS One

Article Title: WNK2 may promote ovarian cancer progression by upregulating POU5F1B

doi: 10.1371/journal.pone.0332003

Figure Lengend Snippet: A, Western blot confirmed the knockdown efficiency of POU5F1B in A2780 and CAOV3 cells. B, and C, CCK8 and colony formation show POU5F1B knockdown suppressed the proliferation of OC cells. D, Transwell assays show POU5F1B knockdown inhibits the migration of OC cells.

Article Snippet: Human OC cell lines CAOV3 and A2780 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Western Blot, Knockdown, Migration

Journal: PLOS One

Article Title: WNK2 may promote ovarian cancer progression by upregulating POU5F1B

doi: 10.1371/journal.pone.0332003

Figure Lengend Snippet: A, Western blot confirmed the overexpression of POU5F1B in A2780 and CAOV3. B and C, CCK8 and colony formation show POU5F1B overexpression promotes the proliferation of OC cells. D, Transwell assays show POU5F1B promotes the migration of OC cells.

Article Snippet: Human OC cell lines CAOV3 and A2780 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Western Blot, Over Expression, Migration

Journal: PLOS One

Article Title: WNK2 may promote ovarian cancer progression by upregulating POU5F1B

doi: 10.1371/journal.pone.0332003

Figure Lengend Snippet: A-B, POU5F1B was successfully overexpressed in the WNK2-knockdown cell lines A2780 and CAOV3. C-D, WNK2 knockdown suppressed the proliferation of OC cells. When POU5F1B was overexpressed, the proliferation inhibition of OC cells was reversed. E-F, POU5F1B promoted the viability of WNK2-knockdown cell in vivo. G, Curves of tumor growth: When visible lumps occurred, tumor volumes were measured every two days. H, Immunohistochemistry staining of POU5F1B in nude mice tumors. WNK2 knockdown inhibited the migration of OC cells. When POU5F1B was overexpressed, the migration inhibition of OC cells was reversed. I, Western blot illustrated the level of p-AKT(473) in OC cells after WNK2 was knocked down and POU5F1B overexpressed.

Article Snippet: Human OC cell lines CAOV3 and A2780 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Knockdown, Inhibition, In Vivo, Immunohistochemistry, Staining, Migration, Western Blot